Drosophila Molecular Genetics

Group leader

Anton Golovnin
Ph.D., Dr.Sc.
Professor of the Russian Academy of Sciences
Group Leader
Lab. of Drosophila Molecular Genetics

Research interests:

drosophila, protein-protein interactions, regulation of transcription,

long distant interactions of the genome regulatory elements, insulator

Information

Analysis of the relationship between the genome architecture and its functioning is a priority research field in molecular genetics. The formation of independent transcriptional domains and spatial contacts between regulatory regions of individual loci or between chromosomes depend on architectural proteins. We study the mechanisms allowing the Drosophila architectural protein Su(Hw) to form complexes that have different regulatory activities, specifically bind to genomic sites, and provide for long-distance interactions between enhancers and promoters.

The Su(Hw)-dependent protein complex is a unique model, since its basic structural components having an effect on its activity are already known. These are the Su(Hw), Mod(mdg4), and CP190 proteins. It is known that the Mod(mdg4)-67.2 isoform interacts only with the Su(Hw) protein. However, the mod(mdg4) locus encodes more than 30 protein isoforms with different C-terminal domains. The role of other Mod(mdg4) isoforms in transcription regulation and organization of chromatic structure remains obscure. We assume that Mod(mdg4) isoforms may facilitate site-specific binding of transcription-regulating protein complexes. Therefore, we are studying the mechanism of interaction between different Mod(mdg4) isoforms and DNA-binding proteins.

In our work we combine the methods of classical genetics (crossing of Drosophila mutants with subsequent genetic and phenotypic analysis of the offspring) with modern methods of molecular biology (CRISPR / Cas9 genome editing, qChIP analysis, RT-PCR, GST Pull Down, etc.).

Group members

Selected publications

Funding

foundation title
RSF Analysis of mechanisms for the specific recruitment of regulatory protein complexes to chromatin and long-distance interactions between them: The example of Drosophila melanogaster Su(Hw) insulator

Additional information

In the laboratory, postgraduate and junior researcher vacancies are open.