Genome Stability

Our long-term research interests involve two major areas: DNA damage response (DDR) and spatial genome organization (3D genome). Research of the group aims to characterize the DDR pathways and components that are activated in response to different cellular stresses. At present, the main focus of the group is studying new DDR pathways operating in the nucleolus and preventing ribosomal DNA instability. From the 3D genome perspective, we are interested in deciphering the role of the so-called weak biological forces such as liquid-liquid phase separation, molecular crowding, RNA environment, etc. in establishing and maintaining the spatial genome organization of the eukaryotic cell. We are also focused on the characterization of new epigenetic drugs capable to modulate gene expression by affecting the 3D genome. Our approach is highly interdisciplinary and relies on both conventional molecular and cellular biology techniques and cutting-edge technologies such as super-resolution microscopy (SIM, STORM, STED), and genome-wide studies (Hi-C, ChIPseq, RNAseq, etc.) and data analysis.

2021 – Treacle and TOPBP1 control replication stress response in the nucleolus ⇒

Velichko AK, Ovsyannikova N, Petrova NV, Luzhin AV, Vorobjeva M, Gavrikov AS, Mishin AS, Kireev II, Razin SV, Kantidze OL.

J Cell Biology 220 (8), e202008085. doi: 10.1083/jcb.202008085

 

2021 – Suppression of liquid-liquid phase separation by 1,6-hexanediol partially compromises the 3D genome organization in living cells. ⇒

Ulianov S.V., Velichko A.K., Magnitov M.D., Luzhin A.V., Golov A.K., Ovsyannikova N., Kireev I.I., Gavrikov A.S., Mishin A.S., Garaev A.K., Tyakht A.V., Gavrilov A.A., Kantidze O.L.*, Razin S.V.*

Nucleic Acids Res. 2021 Apr 9. doi: 10.1093/nar/gkab249.

 

2021 – LASCA: loop and significant contact annotation pipeline. ⇒

Luzhin A.V., Golov A.K., Gavrilov A.A., Velichko A.K., Ulianov S.V., Razin S.V. Kantidze O.L.

Scientific Reports 11, 6361

 

2020 – The 3D genome as a target for anticancer therapy. ⇒

Kantidze O.L., Gurova K.V., Studitsky V.M., Razin S.V.

Trends in Molecular Medicine, 26 (2), 141-149

 

2020 – Weak interactions in higher-order chromatin organization. ⇒

Kantidze O.L., Razin S.V.

Nucleic Acids Res, 48 (9), 4314-4626

 

2019 – Hypoosmotic stress induces R loop formation in nucleoli and ATR/ATM-dependent silencing of nucleolar transcription. ⇒

Velichko A.K., Petrova Nad.V., Luzhin A.V., Ovsyannikova N., Strelkova O.S., Kireev I.I., Petrova Nat.V., Razin S.V., Kantidze O.L.

Nucleic Acids Res, 47 (13), 6811-6825

 

2019 – The anti-cancer drugs curaxins target spatial genome organization. ⇒

Kantidze O.L., Luzhin A.V., Nizovtseva E.V., Safina A., Valieva M.E., Golov A.K., Velichko A.K., Lyubitelev A.V., Feofanov A.V., Gurova K.V., Studitsky V.M., Razin S.V.

Nature Commun, 10 (1), 1441

 

2018 – Synthetically lethal interactions of ATM, ATR, and DNA-PKcs. ⇒

Kantidze O.L., Velichko A.K., Luzhin A.V., Petrova N.V., Razin S.V.

Trends in Cancer, 4 (11), 755-768

 

2016 – Small molecule compounds that induce cellular senescence. ⇒

Petrova N.V., Velichko A.K., Razin S.V., Kantidze O.L.

Aging Cell, 15 (6), 999-1017

 

2015 – Mechanism of heat stress-induced cellular senescence elucidates the exclusive vulnerability of early S-phase cells to mild genotoxic stress. ⇒

Velichko A.K., Petrova N.V., Razin S.V., Kantidze O.L.

Nucleic Acids Research, 43 (13), 6309-6320

 

All papers in 

Foundation	Title
RSF	3D genome structure and stability, and technologies for its targeted modulation for basic research and translational science.
RFBR	The mechanisms of hyperthermia induced cell sensitization to DNA repair enzymes inhibitors